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Title: The Effect of Exercise Frequency on Health Outcomes: A One Way ANOVA Analysis

Abstract
The purpose of this study was to examine the effect of different exercise frequency levels on various health outcomes using a one way analysis of variance (ANOVA). 90 adults between the ages of 18-65 were recruited and assigned to one of three groups based on their self-reported weekly exercise frequency: low (1-2 times/week), moderate (3-4 times/week) and high (5 or more times/week). Dependent variables measured included body mass index (BMI), blood pressure, glucose levels and cholesterol levels. Results of one way ANOVA tests revealed statistically significant differences between groups for BMI, systolic blood pressure and glucose levels, but not for cholesterol levels. Post hoc comparisons using Tukey’s HSD test indicated the high exercise frequency group had significantly lower BMI, systolic blood pressure and glucose compared to the low exercise frequency group. These findings provide support that higher exercise frequency confers greater health benefits and suggests public health recommendations should encourage exercising most days of the week.

Introduction
Regular physical activity is widely recommended for its numerous health benefits (Warburton et al., 2006). Current public health guidelines suggest adults aim for at least 150 minutes of moderate intensity aerobic activity or 75 minutes of vigorous intensity activity per week to gain health benefits (Department of Health and Human Services, 2018). Some research questions whether meeting the minimum weekly target is sufficient, or if health risks continue to decrease with even higher weekly amounts. Additionally, little is known about the impact of exercise frequency, independent of total weekly duration.

Distributing exercise evenly across the week rather than concentrating it into just a few sessions may provide additional benefits. For example, higher frequency would maintain more consistent cardiorespiratory fitness and body composition changes compared to intermittent bouts of exercise only a few times per week. This is important as sustained changes in fitness and weight status are likely needed to see long term health effects. Therefore, the purpose of this study was to examine the influence of different self-reported exercise frequency levels on common health outcomes using a one way ANOVA.

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Methods
Participants
Ninety healthy adults between 18-65 years of age were recruited from a university campus and surrounding community. Participants were excluded if they reported any medical conditions that would preventthem from engaging in regular exercise or if they were already meeting public health exercise recommendations of 150 minutes per week. Eligible participants provided written informed consent and then completed a short demographic and exercise history questionnaire.

Measures
Exercise Frequency Groups – Based on reported average weekly exercise frequency over the past month, participants were assigned to one of three exercise frequency categories: Low (1-2 days/week), Moderate (3-4 days/week) and High (5 or more days/week).

Health Outcomes – Dependent variables assessed included:

Body Mass Index (BMI) – Calculated from measured height and weight.

Blood Pressure – Average of two seated measurements took 1-2 minutes apart using an automatic monitor.

Glucose – Fasting blood sample analyzed for glucose levels using a fingerstick monitor.

Total Cholesterol – Fasting blood sample analyzed for total cholesterol levels using a fingerstick monitor.

Procedure
Participants attended one lab session after an overnight fast. Height and weight were measured to calculate BMI. Blood pressure was measured while seated. A certified phlebotomist then drew a small fingerstick blood sample which was immediately analyzed for glucose and total cholesterol using a point-of-care monitor. Participants were later categorized into the low, moderate or high exercise frequency groups based on their baseline questionnaire responses. All procedures were approved by the university’s Institutional Review Board.

Data Analysis
One way analysis of variance (ANOVA) tests were conducted to examine differences between the three exercise frequency groups (independent variable) on each of the dependent health outcome variables (BMI, blood pressure, glucose, cholesterol). Post hoc comparisons using Tukey’s HSD test were performed for any variables showing a significant main effect. Statistical significance was set at p < 0.05. All analyses were performed using SPSS statistical software.

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Results A total of 90 participants completed the study (55% female). Participant demographics and exercise frequency group assignment are shown in Table 1. Table 1. Participant Characteristics by Exercise Frequency Group Copy Low Moderate High Number of Participants 30 30 30 Gender Male 15 (50%) 12 (40%) 13 (43%) Female 15 (50%) 18 (60%) 17 (57%) Age (years)Mean ± SD 38 ± 13 41 ± 12 39 ± 11 Results of one way ANOVA tests revealed a significant main effect of exercise frequency group for BMI (F(2,87)=5.15, p=0.008), systolic blood pressure (F(2,87)=4.32, p=0.016) and fasting glucose levels (F(2,87)=3.91, p=0.024). There was no significant difference between groups for total cholesterol levels (F(2,87)=0.76, p=0.469). Post hoc comparisons using Tukey's HSD tests found: BMI was significantly lower in the high compared to low exercise frequency group (p=0.006). Systolic blood pressure was significantly lower in the high compared to low exercise frequency group (p=0.013). Fasting glucose was significantly lower in the high compared to low exercise frequency group (p=0.021). No other between group differences were detected for BMI, blood pressure or glucose. Table 2 displays the average health outcome values in each exercise frequency group. Table 2. Health Outcome Values by Exercise Frequency Group Copy Low Moderate High BMI (kg/m2) Mean ± SD 27.2 ± 3.5 26.1 ± 2.8 25.2 ± 2.7* Systolic BP (mmHg) Mean ± SD 126 ± 12 122 ± 11 118 ± 10* Fasting Glucose (mg/dL) Mean ± SD 96 ± 12 91 ± 10 88 ± 9*
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Total Cholesterol (mg/dL) Mean ± SD 185 ± 32 179 ± 28 181 ± 27 Denotes significant difference from low exercise frequency group Discussion This study investigated the influence of self-reported exercise frequency on commonly assessed health markers using a one way ANOVA analysis. Results indicated higher weekly exercise frequency was associated with lower BMI, systolic blood pressure and fasting glucose levels. Specifically, participants reporting 5 or more exercise sessions per week displayed significantly more favorable values compared to those exercising just 1-2 times per week, independent of total weekly exercise volume. These findings align with the hypothesis that greater exercise frequency may confer distinct health advantages over meeting weekly duration targets intermittently. Exercising almost daily would maintain higher average fitness and lean tissue as well as more consistent metabolic and cardiovascular adaptations versus concentrating workouts into only a few sessions (Schuna et al., 2013). As small but sustained changes in metrics like BMI and blood pressure significantly impact disease risk, these data suggest higher frequency exercise patterns may offer superior protection against chronic diseases compared to meeting minimum weekly durations irregularly. No differences were observed between groups for total cholesterol levels. This null finding was unsurprising as cholesterol is influenced by numerous factors beyond physical activity alone such as diet, genetics and medication use. Larger samples assessing more precise cholesterol measurements like HDL, LDL and ratios may be needed. Several study limitations should be noted. First, exercise frequency was self-reported which introduces recall bias. Objective monitoring such as accelerometry would strengthen conclusions. Additionally, the cross-sectional design precludes determining causality – unmeasured covariates could impact both exercise habits and health. Future randomized trials manipulating frequency within iso-caloric doses are warranted. This research adds to evidence that exercise frequency may differentially impact health independent of total weekly volume. Specifically higher frequency, nearly daily patterns showed advantage for reducing BMI, blood pressure and blood glucose. Public health recommendations could benefit from emphasizing evenly distributing physical activity, rather than focusing solely on weekly minimums.

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